How To Calculate Depth Of Field Microscope. Mt is the tube lens magnification factor (if any; The formula for the depth of field is:

Just as in classical photography, depth of field is determined by the distance from the nearest object plane in focus to that of the farthest plane also simultaneously in focus. Example calculations of depth of field (dof) using a simplified formula for dof and using the wavelength (λ) for green light 0.550µm. In microscopy depth of field is very short and usually measured in terms of microns.